Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics

@article{Rawat2011DetrimentalEO,
  title={Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics},
  author={Preeti S. Rawat and Amarjeet Kumar Singh and Krishna Das Ray and Bhupendra Chaudhary and Sanjeev Kumar and Taru Gautam and Shaveta Kanoria and Gurpreet Kaur and Paritosh Kumar and Deepak Pental and Pradeep Kumar Burma},
  journal={Journal of Biosciences},
  year={2011},
  volume={36},
  pages={363-376}
}
High levels of expression of the cry1Ac gene from Bacillus thuringiensis cannot be routinely achieved in transgenic plants despite modifications made in the gene to improve its expression. This has been attributed to the instability of the transcript in a few reports. In the present study, based on the genetic transformation of cotton and tobacco, we show that the expression of the Cry1Ac endotoxin has detrimental effects on both the in vitro and in vivo growth and development of transgenic… CONTINUE READING
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