Deterministic HOX Patterning in Human Pluripotent Stem Cell-Derived Neuroectoderm

  title={Deterministic HOX Patterning in Human Pluripotent Stem Cell-Derived Neuroectoderm},
  author={Ethan S Lippmann and Clay E. Williams and David A Ruhl and Maria Carolina Estevez-Silva and Edwin R Chapman and Joshua J. Coon and Randolph Scott Ashton},
  booktitle={Stem cell reports},
Colinear HOX expression during hindbrain and spinal cord development diversifies and assigns regional neural phenotypes to discrete rhombomeric and vertebral domains. Despite the precision of HOX patterning in vivo, in vitro approaches for differentiating human pluripotent stem cells (hPSCs) to posterior neural fates coarsely pattern HOX expression thereby generating cultures broadly specified to hindbrain or spinal cord regions. Here, we demonstrate that successive activation of fibroblast… CONTINUE READING