Determining the polysaccharide composition of plant cell walls
@article{Pettolino2012DeterminingTP, title={Determining the polysaccharide composition of plant cell walls}, author={Filomena Angela Pettolino and Cherie T Walsh and Geoffrey B. Fincher and Antony Bacic}, journal={Nature Protocols}, year={2012}, volume={7}, pages={1590-1607} }
The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocomposite industries. We present analytical techniques not only to define the fine chemical structures of individual cell wall polysaccharides but also to estimate the overall…
455 Citations
Single-run HPLC Quantification of Plant Cell Wall Monosaccharides.
- BiologyBio-protocol
- 2020
An updated version of this protocol in which all nine cell wall monosaccharides are analyzed in a single high-performance liquid chromatography HPAEC-PAD gradient profile, with the inclusion of an enzymatic starch degradation and alternate internal standards for added quantification accuracy.
Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.
- BiologyMethods in molecular biology
- 2020
This chapter describes a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.
Analytical implications of different methods for preparing plant cell wall material.
- BiologyCarbohydrate polymers
- 2021
Glycan profiling of plant cell wall polymers using microarrays.
- BiologyJournal of visualized experiments : JoVE
- 2012
A microarray-based glycan screening method called Comprehensive Microarray Polymer Profiling (CoMPP) is presented that enables multiple samples to be screened using a miniaturised microarray platform with reduced reagent and sample volumes and is well suited to tracking glycan changes in complex biological systems.
The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis
- BiologyPlants
- 2019
To what extent a selection of organisms from three kingdoms of life might be suitable for the synthesis of plant cell wall polysaccharides is evaluated, by identifying their key attributes for glycoengineering as well as analyzing the glycosidic linkages of their native polymers.
Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues
- Biology, MedicineBiotechnology for Biofuels
- 2017
A side-by-side comparison of the efficacy of four different established glycosyl residue composition analysis methods in the analysis of the glycosy residue composition of cell walls from both dicot (Arabidopsis and Populus) and grass (rice and switchgrass) species is provided.
Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.
- ChemistryAnalytical chemistry
- 2020
The concepts herein developed will facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.
Plant Cell Walls: Impact on Nutrient Bioaccessibility and Digestibility
- Chemistry, MedicineFoods
- 2020
This short narrative review presents an overview of the current knowledge on cell walls and how they impact nutrient bioaccessibility and digestibility.
Pectic Polysaccharides in Plants: Structure, Biosynthesis, Functions, and Applications
- BiologyBiologically-Inspired Systems
- 2019
The large number of pectin-related genes in many plant taxa belies their fundamental importance in evolutionary innovations in plants, and pectins can both facilitate and complicate the use of plant cell walls as feedstocks for useful products and might also possess unique applications in human health and medicine.
Quantitative structural organisation model for wheat endosperm cell walls: Cellulose as an important constituent.
- Chemistry, MedicineCarbohydrate polymers
- 2018
References
SHOWING 1-10 OF 58 REFERENCES
Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases.
- BiologyAnalytical biochemistry
- 2002
It is shown that PACE is a sensitive and simple tool for studying the monosaccharide composition of polysaccharides and of cell wall preparations and in combination with specific hydrolases, it can be used to analyze the structure of polySaccharides.
Plant cell walls: the skeleton of the plant world
- Biology
- 2010
This overview summarises the current state of knowledge in relation to the synthesis and assembly of the wall polysaccharides and touches on an exciting emerging role of the cell wall–plasma membrane–cytoskeleton continuum as a signal perception and transduction pathway allowing plant growth regulation in response to endogenous and exogenous cues.
Whole plant cell wall characterization using solution-state 2D NMR
- BiologyNature Protocols
- 2012
This protocol describes procedures for the preparation and extraction of a biological plant tissue, and presents data that demonstrate the utility of this new NMR whole cell wall characterization procedure with a variety of degradative methods traditionally used for cell wall compositional analysis.
Revealing the structural and functional diversity of plant cell walls.
- Biology, Materials ScienceCurrent opinion in plant biology
- 2008
Morphology and chemical composition of barley endosperm cell walls
- Chemistry
- 1975
Cell walls have been isolated from barley endosperm and found to contain a microfibrillar phase which is embedded in an amorphous matrix. The microfibrillar phase probably consists of cellulose,…
Microanalysis of plant cell wall polysaccharides.
- BiologyMolecular plant
- 2009
Oligosaccharide Mass Profiling enables analysis of wall polymers in Golgi-enriched fractions, the location of nascent matrix polysaccharide biosynthesis, enabling separation of the processes of wall biosynthesis versus post-deposition apoplastic metabolism.
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth.
- BiologyThe Plant journal : for cell and molecular biology
- 1993
This review integrates information on the chemical structure of individual polymers with data obtained from new techniques used to probe the arrangement of the polymers within the walls of individual cells consistent with the physical properties of the wall and its components.
Heterogeneity in the chemistry, structure and function of plant cell walls.
- BiologyNature chemical biology
- 2010
The origins of cell wall heterogeneity are considered and contributing factors that are inherent in the molecular mechanisms of polysaccharide biosynthesis are identified and are crucial for the changing biological functions of the wall during growth and development are identified.
Characterization of the Cell-Wall Polysaccharides of Arabidopsis thaliana Leaves
- Environmental SciencePlant physiology
- 1995
The cell-wall polysaccharides of Arabidopsis thaliana leaves have been isolated, purified, and characterized, and these cell walls are remarkable in that they are particularly rich in phosphate buffer-soluble poly Saccharides.