Determining the polysaccharide composition of plant cell walls

@article{Pettolino2012DeterminingTP,
  title={Determining the polysaccharide composition of plant cell walls},
  author={Filomena Angela Pettolino and Cherie T Walsh and Geoffrey B. Fincher and Antony Bacic},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={1590-1607}
}
The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocomposite industries. We present analytical techniques not only to define the fine chemical structures of individual cell wall polysaccharides but also to estimate the overall… 
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455 Citations
Highly Influential Citations
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Background Citations
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Methods Citations
78
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455 Citations

Single-run HPLC Quantification of Plant Cell Wall Monosaccharides.
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An updated version of this protocol in which all nine cell wall monosaccharides are analyzed in a single high-performance liquid chromatography HPAEC-PAD gradient profile, with the inclusion of an enzymatic starch degradation and alternate internal standards for added quantification accuracy.
Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.
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This chapter describes a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.
Analytical implications of different methods for preparing plant cell wall material.
Glycan profiling of plant cell wall polymers using microarrays.
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A microarray-based glycan screening method called Comprehensive Microarray Polymer Profiling (CoMPP) is presented that enables multiple samples to be screened using a miniaturised microarray platform with reduced reagent and sample volumes and is well suited to tracking glycan changes in complex biological systems.
The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis
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To what extent a selection of organisms from three kingdoms of life might be suitable for the synthesis of plant cell wall polysaccharides is evaluated, by identifying their key attributes for glycoengineering as well as analyzing the glycosidic linkages of their native polymers.
Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues
TLDR
A side-by-side comparison of the efficacy of four different established glycosyl residue composition analysis methods in the analysis of the glycosy residue composition of cell walls from both dicot (Arabidopsis and Populus) and grass (rice and switchgrass) species is provided.
Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.
TLDR
The concepts herein developed will facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.
Plant Cell Walls: Impact on Nutrient Bioaccessibility and Digestibility
TLDR
This short narrative review presents an overview of the current knowledge on cell walls and how they impact nutrient bioaccessibility and digestibility.
Pectic Polysaccharides in Plants: Structure, Biosynthesis, Functions, and Applications
TLDR
The large number of pectin-related genes in many plant taxa belies their fundamental importance in evolutionary innovations in plants, and pectins can both facilitate and complicate the use of plant cell walls as feedstocks for useful products and might also possess unique applications in human health and medicine.
Quantitative structural organisation model for wheat endosperm cell walls: Cellulose as an important constituent.
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