Determining stability of proteins from guanidinium chloride transition curves.

  title={Determining stability of proteins from guanidinium chloride transition curves.},
  author={Faizan Ahmad and Savita Yadav and Suman Taneja},
  journal={The Biochemical journal},
  volume={287 ( Pt 2)},
The guanidinium chloride (GdmCl) denaturation of RNAase A, lysozyme and metmyoglobin was investigated at several pH values by using absorbance measurements at 287, 300 and 409 nm respectively. From these measurements the free-energy change on denaturation, delta Gapp., was calculated, assuming a two-state mechanism, and values of delta Gapp. at zero concentration of the denaturant were measured. For each protein all delta Gapp. values were adjusted to pH 7.00 by using the appropriate… CONTINUE READING


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