Determining restriction digest efficiency using the SNaPshot single-base extension method and CE.

@article{Ballantyne2007DeterminingRD,
  title={Determining restriction digest efficiency using the SNaPshot single-base extension method and CE.},
  author={Kaye N. Ballantyne and Roland A. H. van Oorschot and Manfred Kayser and R. J. Mitchell},
  journal={Electrophoresis},
  year={2007},
  volume={28 10},
  pages={1514-7}
}
Incomplete restriction endonuclease digestion may cause complications during particular applications, where any undigested product may interfere with genotyping accuracy, cloning efficiency or PCR amplification. In some instances it may be useful to measure the amount of undigested DNA remaining in the sample, allowing redigestion or elimination of… CONTINUE READING