Determining Diversity of Freshwater Fungi on Decaying Leaves: Comparison of Traditional and Molecular Approaches

@article{Nikolcheva2003DeterminingDO,
  title={Determining Diversity of Freshwater Fungi on Decaying Leaves: Comparison of Traditional and Molecular Approaches},
  author={Liliya G. Nikolcheva and Amanda M Cockshutt and Felix B{\"a}rlocher},
  journal={Applied and Environmental Microbiology},
  year={2003},
  volume={69},
  pages={2548 - 2554}
}
ABSTRACT Traditional microscope-based estimates of species richness of aquatic hyphomycetes depend upon the ability of the species in the community to sporulate. Molecular techniques which detect DNA from all stages of the life cycle could potentially circumvent the problems associated with traditional methods. Leaf disks from red maple, alder, linden, beech, and oak as well as birch wood sticks were submerged in a stream in southeastern Canada for 7, 14, and 28 days. Fungal biomass, estimated… Expand
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