Determining Annealing Temperatures for Polymerase Chain Reaction

@inproceedings{Porta2012DeterminingAT,
  title={Determining Annealing Temperatures for Polymerase Chain Reaction},
  author={A. Porta and Edward Enners},
  year={2012}
}
  • A. Porta, Edward Enners
  • Published 2012
  • Biology
  • ABSTRACT The polymerase chain reaction (PCR) is a common technique used in high school and undergraduate science teaching. Students often do not fully comprehend the underlying principles of the technique and how optimization of the protocol affects the outcome and analysis. In this molecular biology laboratory, students learn the steps of PCR with an emphasis on primer composition and annealing temperature, which they manipulate to test the effect on successful DNA amplification. Students… CONTINUE READING

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 43 REFERENCES
    Segmentation in the chick embryo hindbrain is defined by cell lineage restrictions
    481
    Note: An algorithm for contour-based region filling
    18
    IAU Colloquium 170 Precise stellar radial velocities
    • 1999
    MR properties of excised neural tissue following experimentally induced inflammation.
    111
    An empirical study of bandwidth predictability in mobile computing
    111
    A New and Fast-Readout Interface for Resistive Chemical Sensors
    36