Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA).

  title={Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA).},
  author={Maria Giulia Battelli and Ada Abbondanza and Silvia Musiani and Laura Buonamici and Paola Strocchi and Pier Luigi Tazzari and Laura Gramantieri and Fiorenzo Stirpe},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  volume={281 1-2},
Xanthine oxidase was purified from human milk and used to immunise rabbits. A competitive immunoenzymatic assay with purified enzyme and rabbit antiserum was optimised to measure xanthine oxidase in human serum, the lowest detectable amount being 0.03 pmol of enzymatic protein. Thus, the test (i) is sensitive enough to determine xanthine oxidase in human serum, being more sensitive than the spectrophotometric method, (ii) it is more convenient for clinical laboratories than other sensitive… CONTINUE READING
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