Determination of water permeability coefficient for human spermatozoa and its activation energy.

@article{Noiles1993DeterminationOW,
  title={Determination of water permeability coefficient for human spermatozoa and its activation energy.},
  author={E E Noiles and Peter Mazur and Paul F Watson and Frederick W. Kleinhans and John K. Critser},
  journal={Biology of reproduction},
  year={1993},
  volume={48 1},
  pages={99-109}
}
Four experiments were conducted to determine the permeability coefficient of human sperm to water (Lp) and its activation energy (Ea). Critical tonicity (tonicity at which 50% of the cells swell and lyse) was determined by equilibrating sperm to 22 degrees C (experiments 1a and 1b), 30, 22, 8, or 0 degrees C (experiment 2a), and 0, -1, -3, -5, or -7 degrees C (experiment 2b) and then exposing them to various hypotonic media (215-3 mOsm). For Lp determination, sperm were equilibrated to 30, 22… CONTINUE READING

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