Determination of vitamin B12 in food products by liquid chromatography/UV detection with immunoaffinity extraction: single-laboratory validation.

@article{CamposGimnez2008DeterminationOV,
  title={Determination of vitamin B12 in food products by liquid chromatography/UV detection with immunoaffinity extraction: single-laboratory validation.},
  author={Esther Campos-Gim{\'e}nez and Patric Fontannaz and Marie-Jos{\'e} Trisconi and Tamara Kilinç and Catherine Gimenez and Pierre Andrieux},
  journal={Journal of AOAC International},
  year={2008},
  volume={91 4},
  pages={786-93}
}
A fast and simple method to determine vitamin B12 in foods is presented. The method allows, in addition to the determination of added cyanocobalamin, the determination of natural vitamin B12 forms, making it also applicable to nonfortified products, especially those that are milk-based. Vitamin B12 is extracted in sodium acetate buffer in the presence of sodium cyanide (100 degrees C, 30 min). After purification and concentration with an immunoaffinity column, vitamin B12 is determined by… CONTINUE READING