Determination of urinary thiocyanate and nitrate using fast ion-interaction chromatography.

@article{Connolly2002DeterminationOU,
  title={Determination of urinary thiocyanate and nitrate using fast ion-interaction chromatography.},
  author={Damian Connolly and Leon Barron and Brett Paull},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2002},
  volume={767 1},
  pages={175-80}
}
A simple and rapid chromatographic method for determination of nitrite, nitrate and thiocyanate is reported, and applied to the analysis of non-, medium and heavy smokers' urine samples. Ion-interaction liquid chromatography was carried out on a short 30 mm x 4.6 mm C18 column (3 microm particle size) with a mobile phase of 10 mM tetrabutylammonium phosphate in 20% MeOH. The chromatography was performed at an elevated temperature of 45 degrees C, at a flow-rate of 1 ml/min. Detection was by… CONTINUE READING
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