Determination of urinary free cortisol by "on-line" liquid chromatography.

  title={Determination of urinary free cortisol by "on-line" liquid chromatography.},
  author={M. Sch{\"o}nesh{\"o}fer and Andreas Kage and Belinda Weber and Ilse Lenz and Eckart K{\"o}ttgen},
  journal={Clinical chemistry},
  volume={31 4},
This is a fully automated method for the specific assessment of urinary free cortisol. A 1-mL urine sample is concentrated and prepurified on a reversed-phase precolumn with alkaline, acid, and organic washes. After selective elution, the cortisol-containing organic eluate is "polarized" by admixing water in such a way that cortisol is focused on the top of a second reversed-phase precolumn. From this precolumn, cortisol is desorbed by backflush, separated from the still-remaining related… CONTINUE READING

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