Determination of unidirectional uptake rates for lipids across the intestinal brush border.

@article{Sallee1972DeterminationOU,
  title={Determination of unidirectional uptake rates for lipids across the intestinal brush border.},
  author={Verney L. Sallee and Frederick A. Wilson and John M. Dietschy},
  journal={Journal of lipid research},
  year={1972},
  volume={13 2},
  pages={184-92}
}
An in vitro method is presented which measures valid, unidirectional uptake rates for lipids across the intestinal brush border. This method combines analysis by a newly devised, double isotope counting system for solubilized tissue with the use of a nonabsorbable marker to correct gross uptake determinations for contamination by adherent mucosal fluid. Of seven markers, only [(3)H]inulin measured adherent mucosal fluid volumes as much as 20% greater than the other markers. Diffusion of the… CONTINUE READING
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