Determination of triamcinolone in human plasma by a sensitive HPLC-ESI-MS/MS method: application for a pharmacokinetic study using nasal spray formulation.

@article{Csar2011DeterminationOT,
  title={Determination of triamcinolone in human plasma by a sensitive HPLC-ESI-MS/MS method: application for a pharmacokinetic study using nasal spray formulation.},
  author={Isabela da Costa C{\'e}sar and Ricardo Martins Duarte Byrro and Fabiana Fernandes de Santana e Silva Cardoso and Iram Moreira Mundim and Leonardo de Souza Teixeira and Weidson Carlo de Sousa and Sandro Ant{\^o}nio Gomes and Karini Bruno Bell{\'o}rio and Juliana Machado Br{\^e}tas and G{\'e}rson Ant{\^o}nio Pianetti},
  journal={Journal of mass spectrometry : JMS},
  year={2011},
  volume={46 3},
  pages={320-6}
}
A liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantitation of triamcinolone in human plasma after nasal spray application was developed and validated. Betamethasone was used as internal standard (IS). The analytes were extracted by a liquid-liquid procedure and separated on a Zorbax Eclipse XDB C(18) column with a mobile phase composed of 2 mM aqueous ammonium acetate pH 3.2 and acetonitrile (55:45). Selected reaction monitoring was… CONTINUE READING