Determination of transferrin-like immunoreactivity in the mucosal homogenate of the duodenum, jejunum, and ileum of normal and iron deficient rats

1. In intestinal mucosal tissue a transferrin-like immunoreactivity (TLIR) was determined after loading with 59Fe-(FeCl3) in vivo. The scraped-off mucosal tissue was homogenized and centrifuged at 100 000×g. The supernatant was fractionated chromatographically. The TLIR was determined by the Mancini-test. 2. Extrapolated to infinite dilutions of the… CONTINUE READING