Determination of thiamine and thiamine phosphates in excitable tissues as thiochrome derivatives by reversed-phase high-performance liquid chromatography on octadecyl silica.

@article{Bontemps1984DeterminationOT,
  title={Determination of thiamine and thiamine phosphates in excitable tissues as thiochrome derivatives by reversed-phase high-performance liquid chromatography on octadecyl silica.},
  author={J. Bontemps and Poehler Philippe and Lucien Bettendorff and Jacques Lombet and G Dandrifosse and E. Schoffeniels and Jacques Crommen},
  journal={Journal of chromatography},
  year={1984},
  volume={307 2},
  pages={283-94}
}
The analysis of thiamine and thiamine phosphates by high-performance liquid chromatography owes its high sensitivity to the fluorescent derivatives or thiochromes obtained by chemical oxidation in alkaline medium. The possibility of performing precolumn oxidation with potassium ferricyanide instead of using the hazardous cyanogen bromide has been investigated. The derivatization step has been optimized with respect to the following parameters: concentration of alkali and oxidant, presence of… CONTINUE READING
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