Determination of the turn-off reaction for the hormone-activated adenylate cyclase.

@article{Cassel1979DeterminationOT,
  title={Determination of the turn-off reaction for the hormone-activated adenylate cyclase.},
  author={Dan Cassel and Fritz Eckstein and Martin Lowe and Zvi Selinger},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 19},
  pages={9835-8}
}
Previous work suggested that hormonal activation of adenylate cyclase involves the introduction of GTP to the regulatory site, and subsequent hydrolysis of the bound GTP terminates the activation. In many tissues the turn-off GTPase reaction cannot be readily measured because of a high background of nonspecific GTP hydrolysis. To circumvent this problem a general assay for the turn-off reaction has now been developed. The adenylate cyclase is first activated by hormone and GTP and the… CONTINUE READING