Determination of the orientational distribution and orientation factor for transfer between membrane-bound fluorophores using a confocal microscope.

@article{Corry2006DeterminationOT,
  title={Determination of the orientational distribution and orientation factor for transfer between membrane-bound fluorophores using a confocal microscope.},
  author={Ben Corry and Dylan Jayatilaka and Boris Martinac and Paul Howard Rigby},
  journal={Biophysical journal},
  year={2006},
  volume={91 3},
  pages={
          1032-45
        }
}
Orientational fluorophores have been a useful tool in physical chemistry, biochemistry, and more recently structural biology due to the polarized nature of the light they emit and that fact that energy can be transferred between them. We present a practical scheme in which measurements of the intensity of emitted fluorescence can be used to determine limits on the mean and distribution of orientation of the absorption transition moment of membrane-bound fluorophores. We demonstrate how… CONTINUE READING

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