Determination of the order of substrate addition to MspI DNA methyltransferase using a novel mechanism-based inhibitor.

@article{Taylor1993DeterminationOT,
  title={Determination of the order of substrate addition to MspI DNA methyltransferase using a novel mechanism-based inhibitor.},
  author={Christopher Michael Taylor and Karen Grady Ford and Bernard A. Connolly and David P Hornby},
  journal={The Biochemical journal},
  year={1993},
  volume={291 ( Pt 2)},
  pages={493-504}
}
The cloning and overexpression of the MspI DNA methyltransferase as a functional fusion with glutathione S-transferase is described. The fusion enzyme retains full biological activity and has been used to investigate the interaction of substrates and inhibitors with MspI DNA methyltransferase. The fusion enzyme has been purified to homogeneity in a single… CONTINUE READING