Determination of the optimal human cell lines for development of human hybridomas.

@article{Abrams1983DeterminationOT,
  title={Determination of the optimal human cell lines for development of human hybridomas.},
  author={Paul G. Abrams and J A Knost and Grace Clarke and Susan Wilburn and Robert K. Oldham and Kenneth A. Foon},
  journal={Journal of immunology},
  year={1983},
  volume={131 3},
  pages={1201-4}
}
Six human cell lines were compared with each other and with murine myeloma NS-1 as to their sensitivity to HAT medium and their ability to form hybrids with human lymphocytes, secret monoclonal immunoglobulin, clone, and maintain detectable levels of monoclonal immunoglobulin secretion for a period of time after fusion. Fusion efficiencies varied from 0 to 50%, and the incidence of immunoglobulin secretion ranged between 1 and 78% of the hybrids. Immunoglobulin secretion of cloned hybrids… CONTINUE READING