Determination of the molar absorptivity of NADH.

@article{Mccomb1976DeterminationOT,
  title={Determination of the molar absorptivity of NADH.},
  author={R. Mccomb and L. Bond and R. Burnett and R. C. Keech and G. Bowers},
  journal={Clinical chemistry},
  year={1976},
  volume={22 2},
  pages={
          141-50
        }
}
The molar absorptivity of NADH at 340 nm has been determined by an indirect procedure in which high-purity glucose is phosphorylated by ATP in the presence of hexokinase, coupled to oxidation of the glucose-6-phosphate by NAD+ in the presence of glucose-6-phosphate dehydrogenase. The average value from 85 independent determinations is 6317 liter mol-1 cm-1 at 25 degrees C and pH 7.8. The overall uncertainty is -4.0 to +5.5 ppt (6292 to 6352 liter mol-1 cm-1), based on a standard error of the… Expand
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