Determination of the minimal amount of Tat activity required for human immunodeficiency virus type 1 replication.

@article{Verhoef1997DeterminationOT,
  title={Determination of the minimal amount of Tat activity required for human immunodeficiency virus type 1 replication.},
  author={Koen Verhoef and Michał Koper and Ben Berkhout},
  journal={Virology},
  year={1997},
  volume={237 2},
  pages={228-36}
}
The Tat protein of human immunodeficiency virus type 1 (HIV-1) is a potent trans-activator of transcription from the viral LTR promoter. Previous mutagenesis studies have identified domains within Tat responsible for binding to its TAR RNA target and for transcriptional activation. The minimal Tat activation domain is composed of the N-terminal 48 residues, and mutational analyses identified a cluster of critical cysteines. The importance of four highly conserved aromatic amino acids within the… CONTINUE READING