Determination of the median toxic dose of type C botulinum toxin in lactating dairy cows.

@article{Moeller2003DeterminationOT,
  title={Determination of the median toxic dose of type C botulinum toxin in lactating dairy cows.},
  author={Robert B. Moeller and Birgit Puschner and Richard L. Walker and Tonie E Rocke and Frank D Galey and James S. Cullor and Alex A Ardans},
  journal={Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc},
  year={2003},
  volume={15 6},
  pages={523-6}
}
Because of the difficulty in identifying botulinum toxin in cattle, it is hypothesized that cattle are sensitive to levels of toxin below the detection limits of current diagnostic techniques (the mouse protection bioassay and the immunostick enzyme-linked immunosorbent assay [ELISA] for type C botulinum toxin). Using an up-down method for toxicologic testing, the median toxic dose (MTD50) for cattle was determined. Four lactating Holstein cows were dosed at 0.125 or 0.25 ng/kg with Clostridium… CONTINUE READING