Determination of the magnitude and enantioselectivity of ligand binding to rat and rabbit serum albumins using immobilized-protein high performance liquid chromatography stationary phases.

@article{Massolini1993DeterminationOT,
  title={Determination of the magnitude and enantioselectivity of ligand binding to rat and rabbit serum albumins using immobilized-protein high performance liquid chromatography stationary phases.},
  author={Gabriella Massolini and Alain Aubry and Alison McGann and Irving W Wainer},
  journal={Biochemical pharmacology},
  year={1993},
  volume={46 7},
  pages={
          1285-93
        }
}
Rat, rabbit and human serum albumins were immobilized on an HPLC stationary phase, and the resulting phases were tested for their abilities to determine the extent and enantioselectivity of ligand binding to the respective albumins. A series of achiral and chiral compounds were chromatographed on the phases including benzodiazepinones, non-steroidal anti-inflammatory drugs, amino acids, warfarin and leucovorin. The chromatographic retentions of the benzodiazepinones and one series of… CONTINUE READING