Determination of the interface of a large protein complex by transferred cross-saturation measurements.

@article{Nakanishi2002DeterminationOT,
  title={Determination of the interface of a large protein complex by transferred cross-saturation measurements.},
  author={Tamiji Nakanishi and Mayumi Miyazawa and Masayoshi Sakakura and Hiroaki Terasawa and Hideo Takahashi and Ichio Shimada},
  journal={Journal of molecular biology},
  year={2002},
  volume={318 2},
  pages={245-9}
}
In an earlier paper, it was shown that the cross-saturation method enables us to identify the contact residues of large protein complexes in a more rigorous manner than is possible using chemical shift perturbation and hydrogen-deuterium exchange experiments. However, there are limitations within the determination of the contact residues by the cross-saturation method, in that the method is difficult to apply to protein complexes with a molecular mass over 150 kDa and/or with weak binding… CONTINUE READING

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