Determination of the human liver UDP-glucuronosyltransferase 2B4 domains involved in the binding of UDP-glucuronic acid using photoaffinity labeling of fusion proteins.

@article{Pillot1993DeterminationOT,
  title={Determination of the human liver UDP-glucuronosyltransferase 2B4 domains involved in the binding of UDP-glucuronic acid using photoaffinity labeling of fusion proteins.},
  author={Thierry Pillot and Mohamed Ouzzine and Sylvie Fournel-Gigleux and C Lafaurie and D Tebbi and S Treat and Anna Radominska and Roger Lester and G{\'e}rard Siest and Jacques Magdalou},
  journal={Biochemical and biophysical research communications},
  year={1993},
  volume={197 2},
  pages={785-91}
}
The interactions between UDP-glucuronic acid and two human liver UDP-glucuronosyltransferase 2B4 peptides (14-150 and 299-446) purified from E. coli as Staphylococcus aureus protein A fusion proteins have been investigated. Photoaffinity labeling with azidonucleotides ([beta-32P]5N3UDP-Glucuronic acid and [beta-32P]5N3UDP-Glucose) and competition experiments with UDP-glucuronic acid and structurally related compounds emphasized the presence of a specific UDP binding site between amino acids 299… CONTINUE READING