Determination of the excited-state lifetimes of the tryptophan residues in barnase, via multifrequency phase fluorometry of tryptophan mutants.

@article{Willaert1992DeterminationOT,
  title={Determination of the excited-state lifetimes of the tryptophan residues in barnase, via multifrequency phase fluorometry of tryptophan mutants.},
  author={K Willaert and R E Loewenthal and Javier Sancho and Matheus Froeyen and A. R. Fersht and Yves Engelborghs},
  journal={Biochemistry},
  year={1992},
  volume={31 3},
  pages={711-6}
}
A multifrequency phase fluorometric study is described for wild-type barnase and engineered mutant proteins in which tryptophan residues have been replaced by less fluorescent residues which do not interfere with the determination of the tryptophan emission spectra and lifetimes. The lifetimes of the three tryptophans in the wild-type protein have been… CONTINUE READING