Determination of the catalytic activity of cyclomaltodextrin glucanotransferase by maltotriose-methylorange assay.

@article{Mkel1988DeterminationOT,
  title={Determination of the catalytic activity of cyclomaltodextrin glucanotransferase by maltotriose-methylorange assay.},
  author={Mika Juhani M{\"a}kel{\"a} and Timo Korpela},
  journal={Journal of biochemical and biophysical methods},
  year={1988},
  volume={15 6},
  pages={307-18}
}
A kinetic assay of cyclomaltodextrin glucanotransferase (EC 2.4.1.19), based on the use of methylorange dye as the indicator of cyclodextrins, was studied and the reaction verified with independent HPLC analyses. The assay was optimized for the enzyme of an alkalophilic Bacillus sp. (ATCC 21783). Described enzymological data provide a reasonable background for detection of possible errors. Numerical correlations with previous assays are presented. This method can be adopted as a standard… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…