Corpus ID: 87814873

Determination of the Titres of AcNPV-CarE with Quantitative PCR and Optimization of the Conditions for Esterase Expression in vitro

@article{Fengqiu2012DeterminationOT,
  title={Determination of the Titres of AcNPV-CarE with Quantitative PCR and Optimization of the Conditions for Esterase Expression in vitro},
  author={An Feng-qiu},
  journal={Journal of Southwest University},
  year={2012}
}
  • An Feng-qiu
  • Published 2012
  • Biology
  • Journal of Southwest University
In order to produce native carboxylesterases(CarEs) protein in vitro in cotton bollworm(Helicoverpa armigera) and to understand its metobolic detoxification of some chemical insecticides such as OPs,synthetic pyrethroids(SPs) and carbamates(CBs),the CarE001H of H.armigera was expressed in Sf9 cells with baculovirus vector from Autographa californica nucleopolyhedrosis virus(AcNPV).The titres of the recombinant virus(AcNPV-CarE) were determined,using quantitative real time PCR(Q-PCR).The… Expand