Determination of the GABA(B) receptor agonist CGP 44532 (3-amino-2-hydroxypropylmethylphosphinic acid) in rat plasma after pre-column derivatization by micro-high-performance liquid chromatography combined with negative electrospray tandem mass spectrometry.

@article{Blum2000DeterminationOT,
  title={Determination of the GABA(B) receptor agonist CGP 44532 (3-amino-2-hydroxypropylmethylphosphinic acid) in rat plasma after pre-column derivatization by micro-high-performance liquid chromatography combined with negative electrospray tandem mass spectrometry.},
  author={Wolfgang Blum and Reiner Aichholz and Philippe Ramstein and J{\"u}rgen K{\"u}hn{\"o}l and Wolfgang Froestl and Sandrine Desrayaud},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={2000},
  volume={748 2},
  pages={349-59}
}
An assay, based on pre-column derivatization and micro-high-performance liquid chromatography-tandem mass spectrometry, was developed for the determination of the GABA(B) agonist CGP 44532 in rat plasma. CGP 44532, a highly polar 3-amino-2(S)-hydroxypropylmethylphosphinic acid, presented difficulties in developing a chromatographic method for the analysis of the compound in rat plasma. Instead of analyzing the target compound directly, it was derivatized prior to separation to a 4… CONTINUE READING