Determination of the DNA-interacting region of the archaebacterial chromosomal protein MC1. Photocrosslinks with 5-bromouracil-substituted DNA.

@article{KatouzianSafadi1991DeterminationOT,
  title={Determination of the DNA-interacting region of the archaebacterial chromosomal protein MC1. Photocrosslinks with 5-bromouracil-substituted DNA.},
  author={M Katouzian-Safadi and Bernard Laine and François J.-M. Chartier and J Y Cremet and D B{\'e}laiche and P. Sauti{\`e}re and Michel Charlier},
  journal={Nucleic acids research},
  year={1991},
  volume={19 18},
  pages={4937-41}
}
Protein MC1 is the major chromosomal protein in methanosarcinaceae. Using photochemical crosslinking on 5-bromouracil-substituted DNA, we identified the region of the protein that interacts with it. This region is located in the C-terminal part of the polypeptide chain, and the crosslinked amino-acids are in the region 74-86. Tryptophan 74 is one of the… CONTINUE READING