Determination of synthesis rate and lifetime of bacterial mRNAs.

  title={Determination of synthesis rate and lifetime of bacterial mRNAs.},
  author={E Baracchini and H. -J. Bremer},
  journal={Analytical biochemistry},
  volume={167 2},
A method has been developed to determine the synthesis rate and lifetime of bacterial mRNAs, either bulk mRNA or specific mRNAs, with a minimum of physiological disturbance. The method uses hybridization of pulse-labeled RNA to specific probes followed by an evaluation based on a computer simulation of the labeling kinetics of different classes of RNA. The method was applied to the determination of bulk mRNA in Escherichia coli growing in glucose minimal medium: 60% of the instantaneous rate of… CONTINUE READING

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