Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses

@article{Watson2007DeterminationOS,
  title={Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses},
  author={Sarah Watson and Sarah K. Mercier and Chris R Bye and John F. Wilkinson and Anthony L Cunningham and Andrew N Harman},
  journal={Virology Journal},
  year={2007},
  volume={4},
  pages={130 - 130}
}
The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in… CONTINUE READING
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