Determination of steady-state levels of oxidative DNA base modifications in mammalian cells by means of repair endonucleases.

@article{Pflaum1997DeterminationOS,
  title={Determination of steady-state levels of oxidative DNA base modifications in mammalian cells by means of repair endonucleases.},
  author={M C Pflaum and Oliver Will and Bernd Epe},
  journal={Carcinogenesis},
  year={1997},
  volume={18 11},
  pages={2225-31}
}
The alkaline elution technique in combination with various repair endonucleases (Fpg protein, endonuclease III, exonuclease III, T4 endonuclease V) was used to quantify steady-state (background) levels of oxidative base modifications in various types of mammalian cells. In human lymphocytes the number of base modifications sensitive to Fpg protein, which include 8-hydroxyguanine, was 0.25 +/- 0.05 per 10(6) base pairs. Even lower levels (0.07 +/- 0.02 per 10(6) bp) were observed in HeLa cells… CONTINUE READING