Determination of soluble antigens of Clostridium botulinum A by chemiluminescent--immunosorbent assay (CLISA).

Abstract

The chemiluminescent--immunosorbent assay (CLISA) was adopted for Cl. botulinum A soluble toxic antigens determination. Luminol (ABEI) labelled botulinum antitoxin globulins showed a strongly positive specific immunochemiluminescent reactions with the native botulinum toxin preparations coupled (adsorbed) on polystyrene balls. The sensitivity of the reaction reached 20 DLM/ml (5,000 light impulses per 40 sec) in comparison with 2 X 10(6) DLM (57,000 impulses), control preparations (1,500 impulses) and the background (150-300 impulses). The results give a perspective further investigations with the CLISA for rapid indication of Cl. botulinum toxic antigens.

Cite this paper

@article{Ligieza1986DeterminationOS, title={Determination of soluble antigens of Clostridium botulinum A by chemiluminescent--immunosorbent assay (CLISA).}, author={Janusz Ligieza and Marta Michalik and Joshua Reiss and Jacek Grzybowski}, journal={Archivum immunologiae et therapiae experimentalis}, year={1986}, volume={34 2}, pages={189-95} }