Determination of serum somatomedin levels in patients with Cushing's syndrome by competitive protein-binding assay


A competitive protein-binding assay has been developed using: 1) a rat liver protein which proved specifically to bind somatomedins (SM) and 2) 125I NSILA-S*as tracer and NSILA-S*(4.5mU/mg) as standard. NSILA-S, SM-A**and serum SM extracted by acidic gel filtration gave parallel displacement curves. Results are expressed in relation to a standard pool of… (More)
DOI: 10.1203/00006450-197910000-00063


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