Determination of serum proteins by means of the biuret reaction.

@article{Gornall1949DeterminationOS,
  title={Determination of serum proteins by means of the biuret reaction.},
  author={Allan G. Gornall and C J Bardawill and M M David},
  journal={The Journal of biological chemistry},
  year={1949},
  volume={177 2},
  pages={
          751-66
        }
}
In the course of an investigation of the biochemical changes following experimental liver injury we felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum. Among the simpler procedures known, the biuret reaction seemed to offer the most encouraging possibilities. Variations and improvements in the application of the biuret reaction to clinical chemistry can be traced in the works of Autenrieth (l), Hiller (2), Fine (3), Kingsley (4… Expand
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