Corpus ID: 17177073

Determination of serum bile acids in hepatobiliary diseases by gas liquid chromatography (fasting serum bile acid levels and endogenous bile acid tolerance test).

@article{Kajiyama1981DeterminationOS,
  title={Determination of serum bile acids in hepatobiliary diseases by gas liquid chromatography (fasting serum bile acid levels and endogenous bile acid tolerance test).},
  author={G. Kajiyama and C. Fukuhara and K. Oyamada and I. Horiuchi and M. Nakagawa and K. Takata and A. Miyoshi},
  journal={Hiroshima journal of medical sciences},
  year={1981},
  volume={30 4},
  pages={
          263-71
        }
}
The methods and results of a gas-liquid chromatographic analysis of bile acids in serum are presented. The analysis of bile acids in serum involves enzymatic hydrolysis (cholylglycine hydrolase), preparation of propionated methyl ester derivatives of bile acid and gas chromatographic procedure with 2. 5 % OV-1. Adequate separation of the individual bile acids, (cholic, chenodeoxycholic and deoxycholic acid) was achieved with vitamine E caprylate as an internal standard. A detector response· was… Expand

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