Determination of semicarbazide-sensitive amine oxidase activity in human plasma by high-performance liquid chromatography with fluorimetric detection.

@article{Dijk1995DeterminationOS,
  title={Determination of semicarbazide-sensitive amine oxidase activity in human plasma by high-performance liquid chromatography with fluorimetric detection.},
  author={Jeroen P. van Dijk and Frans Boomsma and Gooitzen Alberts and A J Man in 't Veld and Maarten A. D. H. Schalekamp},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1995},
  volume={663 1},
  pages={43-50}
}
We report here a simple and sensitive method for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human plasma. Benzaldehyde, generated during a 1-h incubation of plasma with benzylamine, is derivatized with the specific aldehyde reagent dimedone after prior deproteinization. Quantitation of the derivatization product is done by automated injection onto an isocratic high-performance liquid chromatographic system with fluorimetric detection. The assay shows good… CONTINUE READING

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