Determination of selenocysteine and selenomethionine in edible animal tissues by 2D size-exclusion reversed-phase HPLC-ICP MS following carbamidomethylation and proteolytic extraction.

@article{Bierla2008DeterminationOS,
  title={Determination of selenocysteine and selenomethionine in edible animal tissues by 2D size-exclusion reversed-phase HPLC-ICP MS following carbamidomethylation and proteolytic extraction.},
  author={Katarzyna Bierla and Mih{\'a}ly Dernovics and V{\'e}ronique Vacchina and Joanna Szpunar and G{\'e}rard Bertin and Ryszard Lobiński},
  journal={Analytical and bioanalytical chemistry},
  year={2008},
  volume={390 7},
  pages={1789-98}
}
A method was developed for the simultaneous determination of selenomethionine (SeMet) and selenocysteine (SeCys) in meat (chicken and lamb muscles) and different offal tissues (heart, liver, kidney). The analytical procedure was based on the protein extraction with urea under reducing conditions (dithiothreitol), derivatization of SeCys and SeMet by carbamidomethylation with iodoacetamide (IAM) followed by quantitative proteolysis. The mixture of the derivatized Se-amino acids was purified by… CONTINUE READING

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