Determination of selenium in biological matrices.


The definitive IDMS method has the advantage of good detection limits and precision. The advent of the new, smaller, easy-to-use GC/MS system should make this method more popular. The fluorometric method remains the method of choice for the determination of selenium in many matrices if the limitations of the method are understood. The newer methods include gas chromatography, HG-AAS, and GFAAS. The gas chromatography methods show promise because of the relatively simple instrumentation needed and the fact that the analyte is separated from the matrix. HG-AAS offers good sensitivity provided care is taken to ensure complete sample digestion and conversion of selenium to selenite. The advent of Zeeman background correction systems for GFAAS has greatly facilitated selenium determinations, particularly in biological matrices where iron and phosphorus are also present. The reference materials now available, used as part of a quality assurance program, should help to ensure accurate determinations, permit method validation, and allow performance evaluation in interlaboratory trials.

Cite this paper

@article{Lewis1988DeterminationOS, title={Determination of selenium in biological matrices.}, author={Simon A. Lewis}, journal={Methods in enzymology}, year={1988}, volume={158}, pages={391-402} }