Determination of scutellarin in mouse plasma and different tissues by high-performance liquid chromatography.

@article{Xiong2006DeterminationOS,
  title={Determination of scutellarin in mouse plasma and different tissues by high-performance liquid chromatography.},
  author={Fei Xiong and Hao Wang and Ji Emmy Cheng and Jiabi Zhu},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2006},
  volume={835 1-2},
  pages={114-8}
}
A simple HPLC-UV method was developed for the determination of scutellarin in plasma and different tissues of mice (heart, liver, spleen, lungs and kidneys). The separation was achieved by HPLC on a Hypersil C(18) column with a mobile phase composed of methanol-water-glacial acetic acid (40:60:1). UV detection was used at 335 nm. The calibration curves were linear in all matrices (r(2)>0.997) in the concentration range of 0.1-10 microg/ml for plasma and 0.1-20 microg/g for tissue homogenates… CONTINUE READING