Determination of ribonuclease H surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy.

@article{Fang2005DeterminationOR,
  title={Determination of ribonuclease H surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy.},
  author={Shiping Fang and Hye Jin Lee and A W Wark and Hyun Min Kim and Robert M. Corn},
  journal={Analytical chemistry},
  year={2005},
  volume={77 20},
  pages={6528-34}
}
The kinetics of the ribonuclease H (RNase H) surface hydrolysis of RNA-DNA heteroduplexes formed on DNA microarrays was studied using a combination of real-time surface plasmon resonance imaging (SPRI) and surface plasmon fluorescence spectroscopy (SPFS). Time-dependent SPRI and SPFS data at various enzyme concentrations were quantitatively analyzed using a simple model that couples diffusion, enzyme adsorption, and surface enzyme kinetics. This model is characterized by a set of three rate… CONTINUE READING
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