Determination of rat brain kynurenic acid by column-switching HPLC with fluorescence detection.

@article{Fukushima2007DeterminationOR,
  title={Determination of rat brain kynurenic acid by column-switching HPLC with fluorescence detection.},
  author={Takeshi Fukushima and Shigenobu Mitsuhashi and Masayuki Tomiya and Junko Kawai and Kenji Hashimoto and Toshimasa Toyo'oka},
  journal={Biomedical chromatography : BMC},
  year={2007},
  volume={21 5},
  pages={
          514-9
        }
}
Kynurenic acid (KYNA), one of the tryptophan metabolites, serves as an endogenous antagonist of N-methyl-d-aspartate and the alpha7 nicotinic receptors in mammalian brains. In the present study, the column-switching high-performance liquid chromatography (HPLC) method we developed for plasma KYNA was extended and validated for the determination of brain KYNA. Rat cerebrum, cerebellum and brainstem homogenates were deproteinized with acetone, and the extracts reconstituted with the mobile phase… CONTINUE READING