Determination of ractopamine residue in tissues and urine from pig fed meat and bone meal

  title={Determination of ractopamine residue in tissues and urine from pig fed meat and bone meal},
  author={Carolina N Aroeira and Vivian Feddern and Vanessa Gressler and Luciano Molognoni and Heitor Daguer and Osmar A Dalla Costa and Gustavo J M M de Lima and Carmen Josefina Contreras-Castillo},
  journal={Food Additives \& Contaminants: Part A},
  pages={424 - 433}
ABSTRACT In many countries, ractopamine hydrochloride (RAC) is allowed to be used in animal production as a β-agonist, which is an energy repartitioning agent able to offer economic benefits such as increased muscle and decreased fat deposition, feed conversion improvement and an increase in average daily weight gain. However, some countries have banned its use and established strict traceability programmes because of pharmacological implications of β-agonist residues in meat products. In… 

Effects of differing withdrawal times from ractopamine hydrochloride on residue concentrations of beef muscle, adipose tissue, rendered tallow, and large intestine

Results of this study provide a baseline for the development of management protocol recommendations associated with withdrawal following group-feeding of RAC to beef cattle in countries that allow RAC use and intend to export to global markets which may be subject to zero tolerance policies and off-target tissue testing.

Excretion and Residual Concentration Correlations of Salbutamol Between Edible Tissues and Living Samples in Pigs and Goats

Illegal use of salbutamol (SAL), a β-adrenergic leanness-enhancing agent, has posed potential threat to human health in China. The excretion and depletion of SAL in pigs and goats were investigated,

Detection and quantification of residues in sheep exposed to trace levels of dietary zilpaterol HCl

Analytical techniques commonly used to ensure compliance with show-animal rules, import/export guidelines, and regulatory statutes routinely detected residues in animals exposed to zilpaterol at doses insufficient to elicit growth responses.

The determination of β-agonist residues in bovine tissues using liquid chromatography tandem mass spectrometry.

This method was used to detect four β-agonists in 100 bovine muscle, 100 liver, and 100 kidney tissues from a slaughterhouse and no residue was found above the maximum residue limit level.

Current perspective on veterinary drug and chemical residues in food of animal origin

Three key points are discussed: possible sources of drug and chemical residues, human health problems, and the possible method of control and prevention of veterinary drug residues in animal products.

Review of the Analytical Methods Based on HPLC-Electrochemical Detection Coupling for the Evaluation of Organic Compounds of Nutritional and Environmental Interest

This review would like to show the state of the art regarding the coupling of High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection (ED). Since a universal detector for HPLC is

Research on Rapid Detection Technology for β2-Agonists: Multi-Residue Fluorescence Immunochromatography Based on Dimeric Artificial Antigen

The fluorescent lateral flow immunoassay method established in this study could be used as a high-throughput screening for the efficient and sensitive detection of β2-agonists in food.

A novel sensitive electrochemical method for the detection of ractopamine in meat food via polycitrulline-modified electrode

Ractopamine residue levels in spiked beef and mutton samples were detected by the modified electrode and the recovery was in the range of 96%-101%, giving results consistent with a validated HPLC method, providing a new method for detection and quantification of ractopamines residues in animal tissue.



Residue depletion of ractopamine and its metabolites in swine tissues, urine, and serum.

The residue concentrations at withdrawal period of 0 days in all edible tissues were lower than tolerance values established by the FDA and MRL values listed by the JECFA, and support the withdrawal time of0 days established byThe FDA for ractopamine used as feed additive in swine.

Nutritive value of the meat and bone meals from cattle or pigs in broiler diets.

The digestibility of CP was measured by four different in vitro techniques, based on pepsin digestibility, and showed a large variation and did not correlate at all with the in vivo digestibility values.

Comparison of ractopamine accumulation in tissues on days after treatment

Meat quality of swine supplemented with ractopamine under commercial conditions in Brazil.

Results from this study support the recommendation that including 5 mg/kg of RAC in finishing diets should improve live pig performance without detrimental effects on fresh pork quality and cooked pork palatability.

Pharmacokinetics of ractopamine and its organ distribution in rats.

A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was used to investigate the pharmacokinetics and organ distribution of ractopamine in rats and validated results complied with the U.S. Food and Drug Administration's standards.

Monitoring of ractopamine concentration in the mixture of this feed additive with vitamin mineral complex and with swine feed by HPLC

Two suitable HPLC methods were developed and validated for RAC determination in vitamin mineral complex (VMC) and in swineFeed and can be successfully applied for the monitoring of RAC concentrations in samples of VMC and swine feed ensuring economy to producers and security to consumers of swine meat.

Ractopamine analysis in pig kidney, liver and lungs: A validation of the method scope extension using QuEChERS as a sample preparation step.

Identification of ractopamine residues in tissue and urine samples at ultra-trace level using liquid chromatography-positive electrospray tandem mass spectrometry.

Determination of ractopamine in swine, bovine, and turkey tissues by HPLC with fluorescence detection: first action 2011.22.

The method demonstrated acceptable precision and recovery and satisfied the requirements of the AOAC Stakeholder Panel on Veterinary Drug Residue for SLV studies, and the method was adopted Official Methods ofAnalysis First Action 2011.

Laboratory validation of an LC-MS/MS method for the detection of ractopamine, clenbuterol and salbutamol in bovine and swine muscle at sub-μg kg–1 regulatory limits

The method was demonstrated to be suitable for the determination of RAC, CLB and SLB in swine and cattle muscle samples and meets the Brazilian regulatory requirement that establishes criteria and procedures for the determined parameters such as CCα, CCβ, precision and recovery.