• Corpus ID: 248496230

Determination of quantum yield of NADH and FAD in alcohol-water solutions: the analysis of radiative and nonradiative relaxation pathways

@inproceedings{Gorbunova2022DeterminationOQ,
  title={Determination of quantum yield of NADH and FAD in alcohol-water solutions: the analysis of radiative and nonradiative relaxation pathways},
  author={Ioanna A Gorbunova and Marina Danilova and M. E. Sasin and Victor P. Belik and Dmitrii P. Golyshev and Oleg S Vasyutinskii},
  year={2022}
}
Combined studies on fluorescence quantum yield in coenzymes NADH and FAD in water-methanol, water-ethanol and water-propylene glycol mixtures and on time-resolved fluorescence of the same molecules by means of the TCSPC method under excitation at 450 and 355 nm have been carried out. A significant difference in the behavior of NADH and FAD quantum yields in different alcohol-water mixtures was observed. The dependence of quantum yield in NADH on alcohol concentration was found to be similar in… 

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References

SHOWING 1-10 OF 77 REFERENCES
Two-Photon Excited Fluorescence Dynamics in NADH in Water-Methanol Solutions: The Role of Conformation States.
TLDR
An explanation of the heterogeneity in the measured fluorescence decay times in NADH has been suggested based on the influence of the internal molecular electric field in the nicotinamide ring on nonradiative decay rates.
Two-Photon Excited Fluorescence Dynamics in Enzyme-Bound NADH: the Heterogeneity of Fluorescence Decay Times and Anisotropic Relaxation.
TLDR
A comparative analysis of the polarization-insensitive fluorescence decay using multiexponential fitting models has been carried out and suggested the existence of an anisotropic relaxation time of about 1 ns that is related with the rotation of fluorescence transition dipole moment due to the rearrangement of the excited state NADH nuclear configuration.
The role of microenvironment in the mechanisms of fluorescence decay in free and protein-bound NADH
The influence of solution polarity and viscosity on fluorescence decay parameters in free and protein-bound NADH was studied. The fluorescence in NADH dissolved in water, methanol, ethanol, propylene
Time-resolved emission of flavin adenine dinucleotide in water and water-methanol mixtures.
TLDR
The dynamics reveals that within the 4 ps time frame, where most of the nonexponential fluorescence relaxation takes place, the relative motion of the donor-acceptor pair is consistent with a one-dimensional Brownian motion, where the diffusion coefficient and the shape of the confining potential well are solvent dependent.
Optical spectroscopy of nicotinoprotein alcohol dehydrogenase from Amycolatopsis methanolica: a comparison with horse liver alcohol dehydrogenase and UDP-galactose epimerase.
TLDR
The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm and the fluorescence anisotropy decay of enzyme-bound NADH showed no rotational freedom of the NADH cofactor.
Ultrafast Fluorescence Signals from NADH: Resonant Energy Transfer in the Folded and Unfolded Forms.
TLDR
Measurements determine the dynamics of the excited states of both chromophores in NADH by direct excitation of dihydronicotinamide at 380 nm, and adenine at 266 nm and determined that even in what is referred to as an unfolded state in methanol, non-negligible excitation transfer events do take place.
Evidence for an excited-state reaction contributing to NADH fluorescence
TLDR
The fluorescence of reduced β-nicotinamide adenine dinucleotide (NADH) was monitored as a function of the excitation and emission wavelengths and the intensity decay was found to be more heterogeneous than reported earlier.
Conformation of NADH studied by fluorescence excitation transfer spectroscopy.
Sampling the conformation space of FAD in water-methanol mixtures through molecular dynamics and fluorescence measurements.
TLDR
The correlation of the molecular dynamics simulations with fluorescence titration of the FAD in water/methanol mixtures indicates that the internal quenching takes place when the distance between COMs is <5.5 +/- 0.5 A.
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