Determination of pyrimethamine in animal tissue and egg by liquid chromatography with fluorescence detection.

@article{Horii2001DeterminationOP,
  title={Determination of pyrimethamine in animal tissue and egg by liquid chromatography with fluorescence detection.},
  author={Shozo Horii and Masayuki Nishi and Naoya Oku and Hiroshi Miyakawa and Masakatsu Tezuka},
  journal={Journal of AOAC International},
  year={2001},
  volume={84 4},
  pages={1031-4}
}
A sensitive and selective liquid chromatographic (LC) assay was developed to determine the concentration of pyrimethamine in animal tissue and egg by fluorescent derivative. Animal samples were extracted with acetonitrile, centrifuged, and purified by hexane. Fluorescent derivatization was performed by reacting pyrimethamine with chloroacetaldehyde and… CONTINUE READING