Determination of pseudouridine and other nucleosides in human blood serum by high-performance liquid chromatography.

@article{Colonna1983DeterminationOP,
  title={Determination of pseudouridine and other nucleosides in human blood serum by high-performance liquid chromatography.},
  author={Alfredo Colonna and Tommaso Russo and Franca Esposito and Francesco Salvatore and Filiberto Cimino},
  journal={Analytical biochemistry},
  year={1983},
  volume={130 1},
  pages={19-26}
}
A specific, sensitive, and rapid method to measure pseudouridine in human blood serum is described. The method is based on the following steps: (i) deproteinization of serum samples by filtration on membrane cones or by acetonitrile; (ii) purification of nucleosides and concentration of the sample by affinity chromatography on phenylboronate gel followed by lyophilization; and (iii) separation of nucleosides and their quantitation by reverse-phase high-performance liquid chromatography. The… CONTINUE READING

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