Determination of protein-protein interactions of ICIn by the yeast two-hybrid system.


ICln is a ubiquitously expressed eukaryotic protein. Expression of the protein in Xenopus laevis oocytes, the knocking-down of the protein in fibroblasts, or the reconstitution of the protein in lipid bilayer led to the assumption that this protein is an ionic channel or a significant part thereof. However, other possible roles for ICln in potential regulatory mechanisms have been postulated, as diverse as regulator of cell morphology by interacting with the Skb1 protein and/or interaction with core spliceosomal proteins. Here we show that ICln is able to interact with SnRNP core proteins SmD1, SmD2, SmD3, SmX5 and SmB/B'.

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@article{Schmarda2001DeterminationOP, title={Determination of protein-protein interactions of ICIn by the yeast two-hybrid system.}, author={Andreas Schmarda and Fritz Fresser and Martin Gschwentner and Johannes F{\"{u}rst and M. Ritter and Florian Lang and Gerald Baier and Markus Paulmichl}, journal={Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology}, year={2001}, volume={11 1}, pages={55-60} }