Determination of protein in urine by the biuret method.

@article{Hiller1948DeterminationOP,
  title={Determination of protein in urine by the biuret method.},
  author={A. O. Hiller and Roger L. Greif and William W. Beckman},
  journal={The Journal of biological chemistry},
  year={1948},
  volume={176 3},
  pages={
          1421-9
        }
}
A method for the determination of protein in urine by means of the biuret reaction was described by Hiller (1) and by Hiller, McIntosh, and Van Slyke (2), in which pure biuret prepared by Kahlbaum was used as a standard for visual calorimetry. Since Hiller (1) the biuret method has been used for the determination of urinary proteins by Price (3) and by Lehmann (4), and has been widely used in many forms and modifications for the determination of plasma proteins. A review of the literature on… CONTINUE READING

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